In vitro fertilization (IVF) is one of the most popular and effective assisted reproductive methods today. However, the proportion of implantation failure leading to early miscarriage remains high, especially in older women. Preimplantation Genetic Testing for Aneuploidy (PGT-A) would analyze the genetic nature of embryos, screen, select morphologically and genetically normal embryos, reduce the number of embryos in one transfer, and lower the rate of multiple pregnancies and abortion, as well as preventing birth defects. 

The course “APPLICATION OF NGS–ILLUMINA IN PREIMPLANTATION GENETIC TESTING FOR ANEUPLOIDY PGT-A” will provide an overview and help us understand the advantages of embryo screening technology using the next-generation gene-sequencing technology. Students will comprehend the latest genetic disease screening techniques; proficiently perform Preimplantation Genetic Testing for Aneuploidy PGT-A, analyze results, and solve technical problems. 

• Team-based learning: encourage critical thinking, creativity, and proactive learning through group discussions, and group presentations
• On-the-job training: Students observe and practice alongside the lecturers
• Teaching equipment: A 300 m2 Lab with modern equipment from many well-known manufacturers around the world

• Comprehend an overview and understand the principles of the next-generation gene sequencing technology NGS
• Comprehend the purpose and significance of Preimplantation Genetic Testing for Aneuploidy PGT-A 
• Comprehend the next-generation gene sequencing workflow in Preimplantation Genetic Testing for Aneuploidy  
• Implement the procedure for the sample preparation, library preparation, and operation on the Miseq system 
• Proficient in reading and analyzing test results on BlueFuse Multi software 
• Comprehend causes and solutions to common errors during the process 

• An overview of the next-generation gene sequencing technology and its application in Preimplantation Genetic Testing for Aneuploidy PGT-A 
• The principles of the next-generation gene sequencing technology on Illumina's Miseq system and some applications in assisted reproduction 
• The embryo specimens processing procedure with the next-generation gene sequencing technology NGS  
• WGA process. Issues with the quality of WGA samples after extraction and solutions. 
• Library preparation and gene sequencing using Miseq. 
• Analyze results using BlueFuse Multi software on sequenced samples 
• Course summary. Q&A and Certificate awarding ceremony 

• Course Code: SK-01
• Subjects: Doctors, bachelors or engineers specialized in testing in healthcare facilities; bachelors of Biology, Biotechnology, Chemistry 
• Training time: 04 days (8 lessons/day) 
• Number of students: 12 - 16 students per class 
• Course opening: Open every month upon the number of applications 
• Registration package:

- 01 Application form of Phacogen Institute of Technology HERE

- 01 notarized copy of university or college diploma  

- 01 notarized copy of identity card or citizen identification